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Excerpt from Journal of Morphology, 1903, Vol. 18
The small size of the animal makes it available for study alive under compression, and most Of the parts have been examined in this way. Compressed specimens have been killed and fixed, preferably by means of saturated aqueous solution of corrosive. Then hardened in the same uid and stained and mounted whole, and preparations made in this way supplement the live animal. The excretory system in its finer tubes can only be seen in the living specimen. Material was also hardened in various preser vatives for sectioning: Hermann's, Flemming's and Perenyi' fluids were used, also Mayer's picro-nitric, and saturated aqueous corrosive sublimate solution. For general histological purposes I found that the corrosive hardening was the best, followed by iron-haematoxylin stain. I also tried using methylene blue infra vitam. This demonstrated the muscular system beautifully but has not as yet given me satisfaction with the nervous system.
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